Transgenic mouse offspring generated by ROSI     

Pedro MOREIRA  Serafín PéREZ-CEREZALES  Ricardo LAGUNA  Raúl FERNáNDEZ-GONZALEZ  Belén Pintado SANJUANBENITO  Alfonso GUTIéRREZ-ADáN 《The Journal of reproduction and development》2016,62(1):37-42

The production of transgenic animals is an important tool for experimental and applied biology. Over the years, many approaches for the production of transgenic animals have been tried, including pronuclear microinjection, sperm-mediated gene transfer, transfection of male germ cells, somatic cell nuclear transfer and the use of lentiviral vectors. In the present study, we developed a new transgene delivery approach, and we report for the first time the production of transgenic animals by co-injection of DNA and round spermatid nuclei into non-fertilized mouse oocytes (ROSI). The transgene used was a construct containing the human CMV immediate early promoter and the enhanced GFP gene. With this procedure, 12% of the live offspring we obtained carried the transgene. This efficiency of transgenic production by ROSI was similar to the efficiency by pronuclear injection or intracytoplasmic injection of male gamete nuclei (ICSI). However, ICSI required fewer embryos to produce the same number of transgenic animals. The expression of Egfp mRNA and fluorescence of EGFP were found in the majority of the organs examined in 4 transgenic lines generated by ROSI. Tissue morphology and transgene expression were not distinguishable between transgenic animals produced by ROSI or pronuclear injection. Furthermore, our results are of particular interest because they indicate that the transgene incorporation mediated by intracytoplasmic injection of male gamete nuclei is not an exclusive property of mature sperm cell nuclei with compact chromatin but it can be accomplished with immature sperm cell nuclei with decondensed chromatin as well. The present study also provides alternative procedures for transgene delivery into embryos or reconstituted oocytes.  相似文献   

Effects of soil flooding on leaf gas exchange and growth of two neotropical pioneer tree species     

Marcelo?Schramm?MielkeEmail author  Alex-Alan?Furtado?De?Almeida  Fábio?Pinto?Gomes  Pedro?Antonio?Oliveira?Mangabeira  Delmira?Da?Costa?Silva 《New Forests》2005,29(2):161-168

Schinus terebinthifolius Raddi (Anacardiaceae) and Rapanea ferruginea (Ruiz & Pavon) Mez (Myrsinaceae) are two neotropical pioneer trees with wide geographical distribution in South America, highly degree of adaptation to different soil conditions and intense regeneration in areas with anthropic activities. With the aim to recommend the use Schinus and Rapanea in gallery forest restoration programs, we conducted an experiment with the objective to analyze the capacity of these two pioneer trees to tolerate soil flooding, mainly by accessing the effects of flooding on leaf gas exchange, growth and dry matter partitioning. Seedling survival throughout the 56-day flooding period were 100 and 90% for Schinus and Rapanea, respectively. The mean values of stomatal conductance (g_s) and net photosynthesis (A) observed in the control seedlings were, respectively, 0.4 mol m^–2s^–1 and 14 mmolm^–2s^–1, for Schinus, and 0.5 mol m^–2s^–1 and 14 mmolm^–2s^–1, for Rapanea. On day 20 flooding reduced gs and A by 36 and 29% in Schinus, and 81 and 61% in Rapanea. At the end of the experiment, significant decreases were also observed for root and whole plant biomass, in both species. Based on the results, we concluded that seedlings of Schinus and Rapanea can survive and grow throughout a medium period of soil waterlogging, in spite of the alterations observed in their physiological behavior, such as the decreases in stomatal conductance and in whole plant biomass.  相似文献   

Antibacterial activity of Borreria verticillata roots     

de Sá Peixoto Neto PA  Vladimilsa Silva M  Corrente Campos NV  Porfírio Z  Caetano LC 《Fitoterapia》2002,73(6):529-531

Borreria verticillata roots methanolic extract exhibited a broad antibacterial activity against multiresistant strains of Pseudomonas aeruginosa.  相似文献   

An outbreak of sand impaction in postpartum dairy cows          下载免费PDF全文

Melendez P  Krueger T  Benzaquen M  Risco C 《The Canadian veterinary journal. La revue veterinaire canadienne》2007,48(10):1067-1070

Twenty-two cases of indigestion occurred in a 650-cow herd. Five cows had severe sand abomasal impaction, diagnosed by laparotomy. The pH of prepartum cows' urine was < 6.0 and of sand 8.0. Feed showed a dietary cation-anion difference < or = -110 mEq/kg. After feeding management corrections, no more cases were diagnosed.  相似文献   

Influence of the number of hosts and the risk of predation on the foraging behavior of the parasitoid Eretmocerus eremicus     

Luis Enrique Chavarín-Gómez  Pedro Torres-Enciso  Paola Andrea Palmeros-Suárez  Ricardo Ramirez-Romero 《Pest management science》2023,79(11):4208-4218

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Serum concentrations of C-reactive protein, serum amyloid A, and haptoglobin in pigs inoculated with African swine fever or classical swine fever viruses     

Sánchez-Cordón PJ  Cerón JJ  Núñez A  Martínez-Subiela S  Pedrera M  Romero-Trevejo JL  Garrido MR  Gómez-Villamandos JC 《American journal of veterinary research》2007,68(7):772-777

OBJECTIVE: To determine serum concentrations of the selected acute-phase proteins (APPs) haptoglobin, serum amyloid A (SAA), and C-reactive protein (CRP) in pigs experimentally inoculated with classical swine fever (CSF) and African swine fever (ASF) viruses. ANIMALS: 8 crossbred (Large White x Landrace) 10-week-old pigs. PROCEDURES: Pigs were allocated to 2 groups (4 pigs/group). One group was inoculated with the CSF virus Alfort 187 strain, whereas the other groupwas inoculated with the ASF virus Spain 70 isolate. Blood samples were collected at various time points. At the end of the study, pigs were euthanized and a complete necropsy was performed, including histologic and immunohistochemical analyses. RESULTS: Serum concentrations of APPs increased in pigs inoculated with CSF and ASF viruses, which suggested an acute-phase response in the course of both diseases. The most noticeable increase in concentration was recorded for SAA in both groups (up to a 300-fold increase for CSF virus and an approx 40-fold increase for ASF virus), followed by CRP and then haptoglobin, which each had only 3- to 4-fold increases. CONCLUSIONS AND CLINICAL RELEVANCE: Serum concentrations of APPs increased significantly in pigs inoculated with CSF and ASF viruses. However, differences were evident in serum concentrations of the proteins evaluated in this study.  相似文献   

Hemodynamic effects of target-controlled infusion of propofol alone or in combination with a constant-rate infusion of remifentanil in dogs     

Suzane L. Beier  Cláudio R.S. Mattoso  Antonio J.A. Aguiar  Pedro T.G. Vianna  Flavio Massone 《Canadian journal of veterinary research》2015,79(4):309-315

The objective of this study was to evaluate the hemodynamic effects of target-controlled infusion (TCI) of propofol alone or in combination with a constant-rate infusion (CRI) of remifentanil. Six adult dogs were given 2 treatments in a randomized crossover study with a 7-day interval between treatments. Treatment 1 was propofol (P) and treatment 2 was propofol and remifentanil (P-Rem), without any premedication. Propofol was induced using a TCI system with a predicted plasma concentration (Cp) of 6.0 μg/mL. Anesthesia was maintained within the Cp range (0.65 to 3.0 μg/mL) for 120 min and remifentanil was administered at a rate of 0.3 μg/kg body weight (BW) per minute, CRI. Cardiopulmonary variables were recorded before (baseline), during, and 120 min after drug administration. Heart rate (HR) decreased significantly in the P-Rem group (46%) compared with baseline values. In the P-Rem group, the cardiac index (CI) decreased significantly (49% to 58%) and the stroke volume (SV) decreased compared with baseline values. The systemic vascular resistance index (SVRI) increased significantly in the P-Rem group compared with baseline values. There was no difference in mean arterial pressure (MAP) between the groups. Central venous pressure (CVP) and pulmonary artery occlusion pressure (PAOP) significantly increased in the P-Rem group compared with baseline values. In conclusion, the hemodynamic changes observed in this study indicate a compromise of the cardiovascular system, although the dogs in this study were healthy/euvolemic and there was no change in preload. More studies are required in order to evaluate the actual safety of the combination of propofol and remifentanil in patients with reduced cardiac reserve.  相似文献   

Wood-polymer composites prepared by free radical in situ polymerization of methacrylate monomers into fast-growing pinewood     

Mattos  Bruno D.  de Cademartori  Pedro H. G.  Missio  André L.  Gatto  Darci A.  Magalhães  Washington L. E. 《Wood Science and Technology》2015,49(6):1281-1294

Wood Science and Technology - The aim of this study was to evaluate treatability, morphology and mechanical resistance of composites prepared by in situ polymerization of methyl methacrylate on...  相似文献   

Predation and aridity slow down the spread of 21-year-old planted woodland islets in restored Mediterranean farmland     

José M. Rey Benayas  Loreto Martínez-Baroja  Lorenzo Pérez-Camacho  Pedro Villar-Salvador  Karen D. Holl 《New Forests》2015,46(5-6):841-853

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Genetic variability in natural populations of <Emphasis Type="Italic">Paspalum dilatatum</Emphasis> Poir. analyzed by means of morphological traits and molecular markers     

María Victoria García  Pedro A. Balatti  Miguel J. Arturi 《Genetic Resources and Crop Evolution》2007,54(5):935-946

Native species show adaptive traits that are difficult to find in introduced species. The Pampas region in Argentina is a valuable nature reserve of grasses and Paspalum dilatatum Poir. is one of the most important grasses found there. Based on ploidy level and on morphological traits, five biotypes of P. dilatatum have been described. Two of them were included in this study: a tetraploid biotype with sexual reproduction and a pentaploid biotype with apomictic reproduction. We analyzed the genetic diversity in eight native populations from the Salado basin, Argentina, using both quantitative traits and molecular data (RAPD) with these aims: to obtain information of the degree of phenotypic variation in that area, to know which the pattern of distribution of this variation is and to look for association between molecular markers with populational or biotypic differentiation. Cluster analysis based on morphological data grouped the individuals of the different populations by ploidy level. Molecular markers showed the inverse situation because individuals were grouped by geographic origin as opposed to biotype. Moreover, since RAPD did not discriminate between biotypes with sexual or apomictic reproduction, they are probably not associated with mating system. The results let us conclude that polygenic traits such as LP, LBSR, NRT and NSP can discriminate between biotypes and molecular markers such as bands 12, 40, 19 and 46 can be used to discriminate among populations, probably because they detect neutral variation. The article is part of a thesis presented by María Victoria García in partial fulfillment of the requirements for the Doctor’s degree, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, La Plata, Argentina.  相似文献